Biocatalytic synthesis of poly[ε-caprolactone-co-(12-hydroxystearate)] copolymer for sorafenib nanoformulation useful in drug delivery

Nanoformulations can play an important role in the improvement of anticancer drug therapies. The bioavailability sorafenib, which is exclusively applied treatment unresectable hepatocellular carcinoma, may be increased by its incorporation a biocompatible nanoparticulate matrix that capable targeting and controlling release. copolymers ε-caprolactone are emerging biodegradable compounds for delivery applications. In this work, immobilized lipase three native hydrolases, lipase, esterase protease (two them not previously used as polyesterification catalysts) have been studied biocatalysts synthesis oligomers 12-hydroxystearic acid, proving different selectivity regarding polymerization degree, ratio linear cyclic oligomers, insertion fatty acid units polymeric chain. synthesized poly[ε-caprolactone-co-(12-hydroxystearate)] was novel encapsulating copolymer preparation sorafenib-loaded nanocomposites. nanoparticle formulation emulsion-solvent evaporation method optimized particle size encapsulation efficiency. developed nanotherapeutics showed promising release profile cytotoxic effect vitro HepG2 cells.